Aims: The study was performed to estimate transconjunctival penetration of mitomycin C (MMC) to Tenon′s tissue following application over the intact conjunctiva before routine trabeculectomy.Settings and Design: Institution-based case series.Materials and Methods: In 41 eyes of 41 patients, MMC (0.4 mg/ml for 3 min) was applied over the intact conjunctiva before beginning trabeculectomy.Tenon′s capsule directly beneath the site of application was excised during trabeculectomy and was Ski de fond - Accessoires - Fartage homogenized, centrifuged and MMC concentrations were analyzed using high-performance liquid chromatography (HPLC).
Statistical Analysis Used: Statistical analysis was performed using stata0 8.0 version software (STATA Corporation, Houston, TX, USA).In this study, P -values less than 0.05 were considered as statistically significant.Results: The average weight of the sample of Tenon′s tissue excised was 5.
51 ± 4.42 mg (range: 0.9-17.1) Pipe Beveling Machine and the average estimated MMC concentration found to be present in Tenon′s tissue using HPLC was 18.67 ± 32.
36 x 10−6 moles/kg of the tissue (range: 0.38-197.05 x 10−6 ).In 36 of the 41 patients (87.80%), the MMC concentration reached above 2 x 10−6 moles/kg of the tissue concentration required to inhibit human conjunctival fibroblasts.
Conclusions: Mitomycin C does permeate into the subconjunctival tissue after supraconjunctival application for 3 min.Application of MMC over the conjunctiva may be a useful alternative to subconjunctival or subscleral application during routine trabeculectomy and as an adjunct for failing blebs.